Product Name:Human Surface Membrane Immunoglobulin ELISA Kit
Packing:96T
Catalog No.:ELA-E1282h
Gene Name:SmIg
Detect Range:0.312-20 ng/mL
Sensitivity:0.039ng/mL
Target Protein Name:Surface Membrane Immunoglobulin
Alternative Name:SmIg,Surface Membrane Immunoglobulin
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Human Surface Membrane Immunoglobulin ELISA Kit allows for the in vitro quantitative determination of Surface Membrane Immunoglobulin concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Human Surface Membrane Immunoglobulin ELISA Kit has been pre-coated with an Surface Membrane Immunoglobulin antibody specific to Surface Membrane Immunoglobulin .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Surface Membrane Immunoglobulin and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Surface Membrane Immunoglobulin, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Surface Membrane Immunoglobulin in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
