Product Name:Human cutaneous lymphocyte- associated antigen ELISA Kit
Packing:96T
Catalog No.:ELA-E1184h
Gene Name:CLA
Detect Range:0.312-20 ng/mL
Sensitivity:0.039ng/mL
Target Protein Name:cutaneous lymphocyte-associated antigen
Alternative Name:CLA,cutaneous lymphocyte-associated antigen
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Human cutaneous lymphocyte- associated antigen ELISA Kit allows for the in vitro quantitative determination of cutaneous lymphocyte-associated antigen concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Human cutaneous lymphocyte- associated antigen ELISA Kit has been pre-coated with an cutaneous lymphocyte-associated antigen antibody specific to cutaneous lymphocyte-associated antigen .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for cutaneous lymphocyte-associated antigen and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain cutaneous lymphocyte-associated antigen, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of cutaneous lymphocyte-associated antigen in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
