Product Name:Human liver kidney microsome autoantibody ELISA Kit
Packing:96T
Catalog No.:ELA-E1133h
Gene Name:LKM
Detect Range:0.312-20 ng/mL
Sensitivity:0.039ng/mL
Target Protein Name:liver kidney microsome autoantibody
Alternative Name:LKM,liver kidney microsome autoantibody
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Human liver kidney microsome autoantibody ELISA Kit allows for the in vitro quantitative determination of liver kidney microsome autoantibody concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Human liver kidney microsome autoantibody ELISA Kit has been pre-coated with an liver kidney microsome autoantibody antibody specific to liver kidney microsome autoantibody .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for liver kidney microsome autoantibody and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain liver kidney microsome autoantibody, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of liver kidney microsome autoantibody in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
