Human phospho- extracellular signal- regulated kinase ELISA Kit

Human phospho- extracellular signal- regulated kinase ELISA Kit

Product Name:Human phospho- extracellular signal- regulated kinase ELISA Kit
Packing:96T

Catalog No.:ELA-E0930h

Gene Name:pERK

Detect Range:0.156-10 ng/mL

Sensitivity:0.078ng/mL

Target Protein Name:phospho-extracellular signal-regulated kinase

Alternative Name:pERK,phospho-extracellular signal-regulated kinase
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

ELISA type:Sandwich ELISA Kit
Product Description:Human phospho- extracellular signal- regulated kinase ELISA Kit allows for the in vitro quantitative determination of phospho-extracellular signal-regulated kinase concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

ELISA Test Principle:
The microtiter plate provided in Human phospho- extracellular signal- regulated kinase ELISA Kit has been pre-coated with an phospho-extracellular signal-regulated kinase antibody specific to phospho-extracellular signal-regulated kinase .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for phospho-extracellular signal-regulated kinase and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain phospho-extracellular signal-regulated kinase, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of phospho-extracellular signal-regulated kinase in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!