Human Blocking Antibody ELISA Kit

Human Blocking Antibody ELISA Kit

Product Name:Human Blocking Antibody ELISA Kit
Packing:96T

Catalog No.:ELA-E0654h

Gene Name:BA

Detect Range:0.156-10 ng/mL

Sensitivity:0.078ng/mL

Target Protein Name:Blocking Antibody

Alternative Name:BA,Blocking Antibody
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

ELISA type:Sandwich ELISA Kit
Product Description:Human Blocking Antibody ELISA Kit allows for the in vitro quantitative determination of Blocking Antibody concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

ELISA Test Principle:
The microtiter plate provided in Human Blocking Antibody ELISA Kit has been pre-coated with an Blocking Antibody antibody specific to Blocking Antibody .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Blocking Antibody and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Blocking Antibody, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Blocking Antibody in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!