Product Name:Human heat- labile enterotoxin B subunit ELISA Kit
Packing:96T
Catalog No.:ELA-E0491h
Gene Name:LTB
Detect Range:78-5000 pg/mL
Sensitivity:32pg/mL
Target Protein Name:heat-labile enterotoxin B subunit
Alternative Name:LTB,heat-labile enterotoxin B subunit
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Human heat- labile enterotoxin B subunit ELISA Kit allows for the in vitro quantitative determination of heat-labile enterotoxin B subunit concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Human heat- labile enterotoxin B subunit ELISA Kit has been pre-coated with an heat-labile enterotoxin B subunit antibody specific to heat-labile enterotoxin B subunit .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for heat-labile enterotoxin B subunit and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain heat-labile enterotoxin B subunit, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of heat-labile enterotoxin B subunit in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
