Rat ATP1 (ATP synthase subunit alpha, mitochondrial) ELISA Kit (CUSTOM)
96 Tests
Operating instructions
FOR RESEARCH USE ONLY!
Alternative name
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Intended use
Rat ATP1 ELISA kit allows for the in vitro quantitative determination of Rat ATP synthase subunit alpha, mitochondrial concentrations in serum, plasma, tissue homogenates, cell culture supernates, and other biological fluids.
Test principle
Rat ATP1 ELISA kit is based on the competitive binding enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with an antibody specific to ATP1, During the reaction, ATP1 in the sample or standard competes with a fixed amount of biotin-labeled ATP1 for sites on a pre-coated Monoclonal antibody specific to ATP1. Excess conjugate and unbound sample or standard are washed from the plate. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of ATP1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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