Rat Transmembrane protein 132A, TMEM132A ELISA Kit
96 Tests
Operating instructions
FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!
Synonyms
TMEM132A,sGRP78 binding protein,GBP; HSPA5BP1; GRP78-binding protein; HSPA5-binding protein 1; glucose-regulated protein, 78kDa; heat shock 70kDa protein 5 (glucose-regulated protein, 78kDa) binding protein 1; heat shock 70kDa protein 5 binding protein 1; transmembrane protein 132A
Search name
Rat TMEM132A ELIAS KIT ,Rat sGRP78 binding protein ELIAS KIT ,Rat GBP ELIAS KIT ,Rat HSPA5BP1 ELIAS KIT ,Rat GRP78-binding protein ELIAS KIT ,Rat HSPA5-binding protein 1 ELIAS KIT ,Rat glucose-regulated protein ELIAS KIT ,Rat 78kDa ELIAS KIT ,Rat heat shock 70kDa protein 5 ELIAS KIT ,Rat glucose-regulated protein 78kDa binding protein 1 ELIAS KIT ,Rat heat shock 70kDa protein 5 binding protein 1 ELIAS KIT ,Rat transmembrane protein 132A ELIAS KIT
Intended use
This immunoassay kit allows for the in vitro quantitative determination of Rat Transmembrane protein 132A, TMEM132A concentrations in serum, plasma, tissue homogenates, cell culture supernates, and other biological fluids.
Test principle
The ELISA is based on the competitive binding enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with an antibody specific to TMEM132A, During the reaction, TMEM132A in the sample or standard competes with a fixed amount of biotin-labeled TMEM132A for sites on a pre-coated Monoclonal antibody specific to TMEM132A. Excess conjugate and unbound sample or standard are washed from the plate. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of TMEM132A in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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