Rat Purine nucleoside phosphorylase, PNP ELISA Kit
96 Tests
Operating instructions
FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!
Synonyms
PNP,NP,Pnp1,nucleoside phosphorylase,purine nucleoside phosphorylase; PRO1837; PUNP; HEL-S-156an; epididymis secretory sperm binding protein Li 156an; inosine phosphorylase; inosine-guanosine phosphorylase; purine-nucleoside:orthophosphate ribosyltransferase
Search name
Rat PNP ELISA KIT ,Rat NP ELISA KIT ,Rat Pnp1 ELISA KIT ,Rat nucleoside phosphorylase ELISA KIT ,Rat purine nucleoside phosphorylase ELISA KIT ,Rat PRO1837 ELISA KIT ,Rat PUNP ELISA KIT ,Rat HEL-S-156an ELISA KIT ,Rat epididymis secretory sperm binding protein Li 156an ELISA KIT ,Rat inosine phosphorylase ELISA KIT ,Rat inosine-guanosine phosphorylase ELISA KIT ,Rat purine-nucleoside:orthophosphate ribosyltransferase ELISA KIT
Intended use
This immunoassay kit allows for the in vitro quantitative determination of Rat Purine nucleoside phosphorylase, PNP concentrations in serum, plasma, tissue homogenates, cell culture supernates, and other biological fluids.
Test principle
The ELISA is based on the competitive binding enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with an antibody specific to PNP, During the reaction, PNP in the sample or standard competes with a fixed amount of biotin-labeled PNP for sites on a pre-coated Monoclonal antibody specific to PNP. Excess conjugate and unbound sample or standard are washed from the plate. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of PNP in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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