Rat Phosphoglycerate kinase 1, PGK1 ELISA KIT
96 Tests
Operating instruction
FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!
Synonyms
Phosphoglycerate kinase 1,Cell migration-inducing gene 10 protein,Primer recognition protein 2,PRP 2,PGK1,HEL-S-68p; MIG10; PGKA; PRP 2; cell migration-inducing gene 10 protein; epididymis secretory sperm binding protein Li 68p; primer recognition protein 2; phosphoglycerate kinase 1
Search name
Rat Phosphoglycerate kinase 1 ELISA KIT ,Rat Cell migration-inducing gene 10 protein ELISA KIT ,Rat Primer recognition protein 2 ELISA KIT ,Rat PRP 2 ELISA KIT ,Rat PGK1 ELISA KIT ,Rat HEL-S-68p ELISA KIT ,Rat MIG10 ELISA KIT ,Rat PGKA ELISA KIT ,Rat PRP 2 ELISA KIT ,Rat cell migration-inducing gene 10 protein ELISA KIT ,Rat epididymis secretory sperm binding protein Li 68p ELISA KIT ,Rat primer recognition protein 2 ELISA KIT
Intended use
This immunoassay kit allows for the in vitro quantitative determination of Rat Phosphoglycerate kinase 1 concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.
Test principle
The microtiter plate provided in this kit has been pre-coated with an antibody specific to Phosphoglycerate kinase 1. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for Phosphoglycerate kinase 1 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Phosphoglycerate kinase 1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Phosphoglycerate kinase 1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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