Rat DNA fragmentation factor subunit beta, DFFB ELISA Kit
96 Tests
Operating instructions
FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!
Synonyms
Dffb,CAD; CPAN; DFF-40; DFF2; DFF40; DNA fragmentation factor 40 kDa subunit; DNA fragmentation factor subunit beta; caspase-activated DNase; caspase-activated deoxyribonuclease; caspase-activated nuclease; DNA fragmentation factor 40kDa beta polypeptide,caspase-activated DNase
Search name
Rat Dffb ELISA KIT ,Rat CAD ELISA KIT ,Rat CPAN ELISA KIT ,Rat DFF-40 ELISA KIT ,Rat DFF2 ELISA KIT ,Rat DFF40 ELISA KIT ,Rat DNA fragmentation factor 40 kDa subunit ELISA KIT ,Rat DNA fragmentation factor subunit beta ELISA KIT ,Rat caspase-activated DNase ELISA KIT ,Rat caspase-activated deoxyribonuclease ELISA KIT ,Rat caspase-activated nuclease ELISA KIT ,Rat DNA fragmentation factor 40kDa beta polypeptide ELISA KIT ,Rat caspase-activated DNase ELISA KIT
Intended use
This immunoassay kit allows for the in vitro quantitative determination of Rat DNA fragmentation factor subunit beta, DFFB concentrations in serum, plasma, tissue homogenates, cell culture supernates, and other biological fluids.
Test principle
The ELISA is based on the competitive binding enzyme immunoassay technique.The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat DNA fragmentation factor subunit beta, DFFB, During the reaction, Rat DNA fragmentation factor subunit beta, DFFB in the sample or standard competes with a fixed amount of biotin-labeled Rat DNA fragmentation factor subunit beta, DFFB for sites on a pre-coated Monoclonal antibody specific to Rat DNA fragmentation factor subunit beta, DFFB. Excess conjugate and unbound sample or standard are washed from the plate. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Rat DNA fragmentation factor subunit beta, DFFB in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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