Rat Triosephosphate Isomerase 1 ELISA试剂盒

Rat Triosephosphate isomerase,TPI1 ELISA Kit

96 Tests

Operating instruction

 

FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!

 

Synonyms

TPI1,triosephosphate isomerase 1; triosephosphate isomerase; HEL-S-49; TIM; TPI; TPID; epididymis secretory protein Li 49; triose-phosphate isomerase

 

Search name

Rat TPI1 ELISA KIT ,Rattriosephosphate isomerase 1 ELISA KIT ,Rat triosephosphate isomerase ELISA KIT ,Rat HEL-S-49 ELISA KIT ,Rat TIM ELISA KIT ,Rat TPI ELISA KIT ,Rat TPID ELISA KIT ,Rat epididymis secretory protein Li 49 ELISA KIT ,Rat triose-phosphate isomerase ELISA KIT

 

Intended use

This immunoassay kit allows for the in vitro quantitative determination of Rat triosephosphate isomerase,TIM concentrations in serum, Plasma, Urine, tissue homogenates and Cell culture supernates and Other biological fluids.

 

Test principle

The microtiter plate provided in this kit has been pre-coated with an antibody specific to TIM. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for TIM and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain TIM, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of TIM in the samples is then determined by comparing the O.D. of the samples to the standard curve.

 

 

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