Rat terminal complement complex C5b-9,TCC C5b-9 ELISA Kit
96 Tests
Operating instructions
FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!
Synonyms
terminal complement complex C5b-9,TCC C5b-9, C5b-9, C5b9, TCC C5b9,
Search name
Rat terminal complement complex C5b-9 ELISA KIT ,Rat TCC C5b-9 ELISA KIT ,Rat C5b-9 ELISA KIT ,Rat C5b9 ELISA KIT ,Rat TCC C5b9 ELISA KIT
Intended use
This immunoassay kit allows for the in vitro quantitative determination of Rat terminal complement complex C5b-9,TCC C5b-9 concentrations in serum, plasma, tissue homogenates, cell culture supernates, and other biological fluids.
Test principle
The ELISA is based on the competitive binding enzyme immunoassay technique.The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat terminal complement complex C5b-9,TCC C5b-9, During the reaction, Rat terminal complement complex C5b-9,TCC C5b-9 in the sample or standard competes with a fixed amount of biotin-labeled Rat terminal complement complex C5b-9,TCC C5b-9 for sites on a pre-coated Monoclonal antibody specific to Rat terminal complement complex C5b-9,TCC C5b-9. Excess conjugate and unbound sample or standard are washed from the plate. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Rat terminal complement complex C5b-9,TCC C5b-9 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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