Product Name:Bovine Platelet- activating factor receptor, PTAFR ELISA KIT
Packing:96T
Catalog No.:ELI-45457b
Gene Name:Bovine Platelet-activating factor receptor, PTAFR
Detect Range:0.313-20ng/ml
Sensitivity:0.188ng/ml
Target Protein Name:Bovine Platelet-activating factor receptor, PTAFR
Alternative Name:PTAFR,Bovine Platelet-activating factor receptor, PTAFR
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Bovine Platelet- activating factor receptor, PTAFR ELISA KIT allows for the in vitro quantitative determination of Bovine Platelet-activating factor receptor, PTAFR concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Bovine Platelet- activating factor receptor, PTAFR ELISA KIT has been pre-coated with an Bovine Platelet-activating factor receptor, PTAFR antibody specific to Bovine Platelet-activating factor receptor, PTAFR .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Bovine Platelet-activating factor receptor, PTAFR and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Bovine Platelet-activating factor receptor, PTAFR, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Bovine Platelet-activating factor receptor, PTAFR in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
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