Bovine Pituitary- specific positive transcription factor 1, POU1F1 ELISA KIT
96 Tests
Operating instruction
FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!
Synonyms
POU1F1,CPHD1; GHF-1; PIT1; POU1F1a; Pit-1; POU domain, class 1, transcription factor 1; growth hormone factor 1; pituitary-specific positive transcription factor 1; POU class 1 homeobox 1
Search name
Bovine POU1F1 ELISA KIT ,Bovine CPHD1 ELISA KIT ,Bovine GHF-1 ELISA KIT ,Bovine PIT1 ELISA KIT ,Bovine POU1F1a ELISA KIT ,Bovine Pit-1 ELISA KIT ,Bovine POU domain ELISA KIT ,Bovine class 1 ELISA KIT ,Bovine transcription factor 1 ELISA KIT ,Bovine growth hormone factor 1 ELISA KIT ,Bovine pituitary-specific positive transcription factor 1 ELISA KIT ,Bovine POU class 1 homeobox 1 ELISA KIT
Intended use
This immunoassay kit allows for the in vitro quantitative determination of Bovine Pituitary- specific positive transcription factor 1, POU1F1 concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.
Test principle
The microtiter plate provided in this kit has been pre-coated with an antibody specific to Pituitary-specific positive transcription factor 1. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for Pituitary-specific positive transcription factor 1 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Pituitary-specific positive transcription factor 1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Pituitary-specific positive transcription factor 1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Customized product
Delivery time 4-6month
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