Product Name:Bovine Extracellular calcium- sensing receptor, CASR ELISA KIT
Packing:96T
Catalog No.:ELI-04987b
Gene Name:Bovine Extracellular calcium-sensing receptor, CASR
Detect Range:31.2-2000pg/ml
Sensitivity:18.75pg/ml
Target Protein Name:Bovine Extracellular calcium-sensing receptor, CASR
Alternative Name:CASR,Bovine Extracellular calcium-sensing receptor, CASR
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Bovine Extracellular calcium- sensing receptor, CASR ELISA KIT allows for the in vitro quantitative determination of Bovine Extracellular calcium-sensing receptor, CASR concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Bovine Extracellular calcium- sensing receptor, CASR ELISA KIT has been pre-coated with an Bovine Extracellular calcium-sensing receptor, CASR antibody specific to Bovine Extracellular calcium-sensing receptor, CASR .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Bovine Extracellular calcium-sensing receptor, CASR and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Bovine Extracellular calcium-sensing receptor, CASR, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Bovine Extracellular calcium-sensing receptor, CASR in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
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