Product Name:Bovine Tumor necrosis factor Beta,TNF- Beta ELISA Kit
Packing:96T
Catalog No.:ELA-E0134b
Gene Name:Bovine Tumor necrosis factor Beta,TNF-Beta
Detect Range:0.781-50ng/ml
Sensitivity:0.469ng/ml
Target Protein Name:Bovine Tumor necrosis factor Beta,TNF-Beta
Alternative Name:TNF-Beta,Bovine Tumor necrosis factor Beta,TNF-Beta
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Bovine Tumor necrosis factor Beta,TNF- Beta ELISA Kit allows for the in vitro quantitative determination of Bovine Tumor necrosis factor Beta,TNF-Beta concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Bovine Tumor necrosis factor Beta,TNF- Beta ELISA Kit has been pre-coated with an Bovine Tumor necrosis factor Beta,TNF-Beta antibody specific to Bovine Tumor necrosis factor Beta,TNF-Beta .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Bovine Tumor necrosis factor Beta,TNF-Beta and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Bovine Tumor necrosis factor Beta,TNF-Beta, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Bovine Tumor necrosis factor Beta,TNF-Beta in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
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