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Cluster of differentiation 40 ligand, CD40L ELISA Kit/ Mouse Cluster of differentiation 40 ligand, CD40L ELISA试剂盒

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  • Mouse Cluster of differentiation 40 ligand, CD40L ELISA Kit
    96 Tests
    Operating instruction

    FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE
    BEGINNING!

    Synonyms
    CD40 ligand(CD40-L),Tumor necrosis factor ligand superfamily member 5,T-cell antigen Gp39,TNF-related activation protein(TRAP),
    CD40 ligand,CD154; CD40L; HIGM1; IGM; IMD3; T-BAM; TNFSF5; TRAP; gp39; hCD40L; CD40 antigen ligand; CD40-L; T-B
    cell-activating molecule;

    Search name
    Mouse CD40 ligand ELISA Kit, Mouse CD40-L ELISA Kit, Mouse Tumor necrosis factor ligand superfamily member 5 ELISA Kit,
    Mouse Cluster of differentiation 40 ligand ELISA Kit, Mouse CD40L ELISA Kit, Mouse T-cell antigen Gp39 ELISA Kit, Mouse
    TNF-related activation protein ELISA Kit, Mouse TRAP ELISA Kit, Mouse CD154 ELISA Kit, Mouse HIGM1 ELISA Kit, Mouse IGM
    ELISA Kit, Mouse IMD3 ELISA Kit, Mouse T-BAM ELISA Kit, Mouse TNFSF5 ELISA Kit, Mouse gp39 ELISA Kit, Mouse hCD40L
    ELISA Kit, Mouse CD40 antigen ligand ELISA Kit, Mouse T-B cell-activating molecule ELISA Kit

    Intended use
    This immunoassay kit allows for the in vitro quantitative determination of mouse cd40 ligand,CD40-L concentrations in serum, Plasma,
    tissue homogenates and Cell culture supernates and Other biological fluids.

    Test principle
    The microtiter plate provided in this kit has been pre-coated with an antibody specific to CD40-L. Standards or samples are then added to
    the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for CD40-L and Avidin conjugated
    to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well.
    Only those wells that contain CD40-L, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The
    enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured
    spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of CD40-L in the samples is then determined by comparing
    the O.D. of the samples to the standard curve.

    PDF manual download
     

     


     

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