Mouse Epidermal growth factor, EGF ELISA Kit
96 Tests
Operating instruction
FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE
BEGINNING!
Synonyms
Pro-epidermal growth factor(EGF), HOMG4; URG; beta-urogastrone; pro-epidermal growth factor; epidermal growth factor
Search name
Mouse Pro-epidermal growth factor,EGF ELISA Kit, Mouse EGF ELISA Kit, Mouse HOMG4 ELISA Kit, Mouse URG ELISA Kit,
Mouse beta-urogastrone ELISA Kit, Mouse Epidermal growth factor ELISA Kit,
Intended use
This immunoassay kit allows for the in vitro quantitative determination of mouse pro-epidermal growth factor,EGF concentrations in
serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.
Test principle
The microtiter plate provided in this kit has been pre-coated with an antibody specific to EGF. Standards or samples are then added to the
appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for EGF and Avidin conjugated to
Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well.
Only those wells that contain EGF, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The
enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured
spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of EGF in the samples is then determined by comparing the
O.D. of the samples to the standard curve.
PDF Manual download
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