Mouse Plasminogen activator inhibitor 1,PAI ELISA Kit
96 Tests
Operating instruction
FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!
Synonyms
Plasminogen activator inhibitor 1,PAI,PAI-1, Mr1,Pai1,Planh1,Endothelial plasminogen activator inhibitor,Serpin E1, Serpine1,PAI-1,PAI; PAI1; PLANH1; endothelial plasminogen activator inhibitor; plasminogen activator inhibitor 1; serineproteinase inhibitor clade E member 1; serpin E1; serpin peptidase inhibitor nexin plasminogen activator inhibitor type 1 member 1
Search name
Mouse Plasminogen activator inhibitor 1 ELISA KIT ,Mouse PAI ELISA KIT ,Mouse PAI-1 ELISA KIT ,Mouse Mr1 ELISA KIT ,Mouse Pai1 ELISA KIT ,Mouse Planh1 ELISA KIT ,Mouse Endothelial plasminogen activator inhibitor ELISA KIT ,Mouse Serpin E1 ELISA KIT ,Mouse Serpine1 ELISA KIT ,Mouse PAI-1 ELISA KIT ,Mouse PAI ELISA KIT ,Mouse PAI1 ELISA KIT ,Mouse PLANH1 ELISA KIT ,Mouse endothelial plasminogen activator inhibitor ELISA KIT ,Mouse plasminogen activator inhibitor 1 ELISA KIT ,Mouse serineproteinase inhibitor clade E member 1 ELISA KIT ,Mouse serpin E1 ELISA KIT ,Mouse serpin peptidase inhibitor nexin plasminogen activator inhibitor type 1 member 1 ELISA KIT
Intended use
This immunoassay kit allows for the in vitro quantitative determination of mouse plasminogen activator inhibitor 1,PAI concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.
Test principle
The microtiter plate provided in this kit has been pre-coated with an antibody specific to PAI. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for PAI and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain PAI, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of PAI in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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