Mouse Granulocyte macrophage colony stimulating factor, GM- CSF ELISA Kit
96 Tests
Operating instruction
FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!
Synonyms
Granulocyte-macrophage colony-stimulating factor,GM-CSF,Colony-stimulating factor,CSF, CSF2,GM-CSF,GMCSF; CSF; granulocyte-macrophage colony-stimulating factor; molgramostin; sargramostim; colony stimulating factor 2,granulocyte-macrophage
Search name
Mouse Granulocyte-macrophage colony-stimulating factor ELISA KIT ,Mouse GM-CSF ELISA KIT ,Mouse Colony-stimulating factor ELISA KIT ,Mouse CSF ELISA KIT ,Mouse CSF2 ELISA KIT ,Mouse GM-CSF ELISA KIT ,Mouse GMCSF ELISA KIT ,Mouse CSF ELISA KIT ,Mouse granulocyte-macrophage colony-stimulating factor ELISA KIT ,Mouse molgramostin ELISA KIT ,Mouse sargramostim ELISA KIT ,Mouse colony stimulating factor 2 ELISA KIT ,Mouse granulocyte-macrophage ELISA KIT
Intended use
This immunoassay kit allows for the in vitro quantitative determination of mouse granulocyte-macrophage colony-stimulating factor,GM-CSF concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.
Test principle
The microtiter plate provided in this kit has been pre-coated with an antibody specific to GM-CSF. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for GM-CSF and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain GM-CSF, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of GM-CSF in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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