Mouse Immunoglobulin G, IgG ELISA Kit
96 Tests
Operating instruction
FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!
Synonyms
Immunoglobulin Y, Immunoglobulin G, IgG,IgY, Immunoglobulin gamma
Search name
Mouse IgG ELISA Kit, Mouse Immunoglobulin G ELISA Kit, Mouse IgY ELISA Kit, Mouse Immunoglobulin Y ELISA Kit, Mouse Immunoglobulin gamma ELISA Kit
Intended use
This immunoassay kit allows for the in vitro quantitative determination of mouse Immunoglobulin G concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.
Test principle
The microtiter plate provided in this kit has been pre-coated with an antibody specific to Immunoglobulin G. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated monoclonal antibody preparation specific for Immunoglobulin G and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Immunoglobulin G, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Immunoglobulin G in the samples is then determined by comparing the O.D. of the samples to the standard curve.
PDF manual download
武汉维克赛思科技有限公司
地址：武汉东湖新技术开发区东信路光谷创业街10栋
联系人：童经理
联系电话：13118103892
Email: info@vecscience.com
微信：13118103892
QQ: 3128136151
