Mouse Electron transfer flavoprotein-ubiquinone oxidoreductase mitochondrial, ETFDH ELISA Kit
96 Tests
Operating instruction
FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!
Synonyms
Class1.5.5.1,Electron transfer flavoprotein-ubiquinone oxidoreductase, mitochondrial(ETF-QO,ETF-ubiquinone oxidoreductase),;
Electron-transferring-flavoprotein dehydrogenase(ETF dehydrogenase),ETFDH,electron transfer flavoprotein ubiquinone oxidoreductase,ETFQO; MADD; ETF dehydrogenase; ETF-QO; ETF-ubiquinone oxidoreductase; electron transfer flavoprotein-ubiquinone oxidoreductase, mitochondrial; electron-transferring-flavoprotein dehydrogenase
Search name
Mouse Class1.5.5.1 ELISA KIT ,Mouse Electron transfer flavoprotein-ubiquinone oxidoreductase mitochondrial ELISA KIT ,Mouse ETF-QO ELISA KIT ,Mouse ETF-ubiquinone oxidoreductase ELISA KIT ,Mouse
Electron-transferring-flavoprotein dehydrogenase ELISA KIT ,Mouse ETF dehydrogenase ELISA KIT ,Mouse ETFDH ELISA KIT ,Mouse electron transfer flavoprotein ubiquinone oxidoreductase ELISA KIT ,Mouse ETFQO ELISA KIT ,Mouse MADD ELISA KIT ,Mouse ETF dehydrogenase ELISA KIT ,Mouse ETF-QO ELISA KIT ,Mouse ETF-ubiquinone oxidoreductase ELISA KIT ,Mouse electron transfer flavoprotein-ubiquinone oxidoreductase mitochondrial ELISA KIT ,Mouse electron-transferring-flavoprotein dehydrogenase ELISA KIT
Intended use
This immunoassay kit allows for the in vitro quantitative determination of Mouse electron transfer flavoprotein-ubiquinone oxidoreductase, mitochondrial,ETF-QO concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.
Test principle
The microtiter plate provided in this kit has been pre-coated with an antibody specific to ETF-QO. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for ETF-QO and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain ETF-QO, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of ETF-QO in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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