advanced glycation end products, AGEs ELISA Kit/ Mouse advanced glycation end products, AGEs ELISA试剂盒

Mouse advanced glycation end products, AGEs ELISA Kit

96 Tests

Operating instruction

FOR RESEARCH USE ONLY!

 

Alternative name

Mouse AGES ELISA KIT ,Mouse AGE ELISA KIT ,Mouse  Advanced glycation end-products ELISA KIT ,Mouse Advanced glycation end product ELISA KIT

 

Intended use

Mouse AGES ELISA KIT allows for the in vitro quantitative determination of mouse Advanced glycation end-products concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.

 

Test principle

The microtiter plate provided in Mouse AGES ELISA KIT has been pre-coated with an antibody specific to Advanced glycation end-product. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for Advanced glycation end-product and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Advanced glycation end-product, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Advanced glycation end-product in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Range 0.78-50ng/ml

Mouse AGES ELISA KIT Manual download

Order Mouse AGES ELISA KIT by email:sales@lifescience-market.com

 

 

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