Mouse Interferon regulatory factor 2- binding protein 2, IRF2BP2 ELISA KIT
96 Tests
Operating instructions
FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!
Synonyms
IRF2BP2, IRF-2BP2,IRF-2-binding protein 2; interferon regulatory factor 2-binding protein 2; interferon regulatory factor 2 binding protein 2
Search name
Mouse IRF2BP2 ELISA KIT ,Mouse IRF-2BP2 ELISA KIT ,Mouse IRF-2-binding protein 2 ELISA KIT ,Mouse interferon regulatory factor 2-binding protein 2 ELISA KIT ,Mouse interferon regulatory factor 2 binding protein 2 ELISA KIT
Intended use
This immunoassay kit allows for the in vitro quantitative determination of mouseIRF-2-binding protein 2concentrations in serum, plasma, tissue homogenates, cell culture supernates, and other biological fluids.
Test principle
The ELISA is based on the competitive binding enzyme immunoassaytechnique. The microtiter plate provided in this kit has been pre-coated with an antibody specific toIRF-2-binding protein 2, During the reaction,IRF-2-binding protein 2in the sample or standard competes with a fixed amount of biotin-labeledIRF-2-binding protein 2 for sites on a pre-coated Monoclonal antibody specific to IRF-2-binding protein 2. Excess conjugate and unbound sample or standard are washed from the plate. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Thena TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of IRF-2-binding protein 2 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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