Mouse All- trans- retinol 13,14- reductase, Retsat ELISA KIT Product Name:Mouse All- trans- retinol 13,14- reductase, Retsat ELISA KIT
Packing:96T
Catalog No.:ELI-52640m
Gene Name:Mouse Retsat
Detect Range:0.15-10ng/ml
Sensitivity:0.094ng/ml
Target Protein Name:Mouse Retsat
Alternative Name:Mouse All-trans-retinol 13,14-reductase, Retsat
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Mouse All- trans- retinol 13,14- reductase, Retsat ELISA KIT allows for the in vitro quantitative determination of Mouse Retsat concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Mouse All- trans- retinol 13,14- reductase, Retsat ELISA KIT has been pre-coated with an Mouse All-trans-retinol 13,14-reductase, Retsat antibody specific to Mouse Retsat .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Mouse Retsat and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Mouse Retsat , biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Mouse Retsat in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
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