Mouse Spermatogenesis- associated serine- rich protein 2, SPATS2 ELISA KIT
96 Tests
Operating instruction
FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!
Synonyms
SPATS2,P59SCR; SCR59; SPATA10; serine-rich spermatocytes and round spermatid 59 kDa protein; serine-rich spermatocytes and round spermatid protein; spermatogenesis-associated serine-rich protein 2; spermatogenesis associated, serine-rich 2
Search name
Mouse SPATS2 ELISA KIT ,Mouse P59SCR ELISA KIT ,Mouse SCR59 ELISA KIT ,Mouse SPATA10 ELISA KIT ,Mouse serine-rich spermatocytes and round spermatid 59 kDa protein ELISA KIT ,Mouse serine-rich spermatocytes and round spermatid protein ELISA KIT ,Mouse spermatogenesis-associated serine-rich protein 2 ELISA KIT ,Mouse spermatogenesis associated serine-rich 2 ELISA KIT
Intended use
This immunoassay kit allows for the in vitro quantitative determination of Mouse Spermatogenesis- associated serine- rich protein 2, SPATS2 concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.
Test principle
The microtiter plate provided in this kit has been pre-coated with an antibody specific to SPATS2. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for SPATS2 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain SPATS2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of SPATS2 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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