Mouse Complement component C8 beta chain,C8B ELISA Kit
96 Tests
Operating instruction
FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!
Synonyms
Complement component C8 beta chain,Complement component 8 subunit beta,CO8B, C82,complement component 8 beta polypeptide
Search name
Mouse Complement component C8 beta chain ELISA Kit, Mouse Complement component 8 subunit beta ELISA Kit, Mouse CO8B ELISA Kit, Mouse C82 ELISA Kit, Mouse complement component 8 beta polypeptide ELISA Kit
Intended use
This immunoassay kit allows for the in vitro quantitative determination of Mouse complement component c8 beta chain,CO8B concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.
Test principle
The microtiter plate provided in this kit has been pre-coated with an antibody specific to CO8B. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for CO8B and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain CO8B, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of CO8B in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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