Mouse Carboxypeptidase N catalytic chain, Cpn1 ELISA KIT Product Name:Mouse Carboxypeptidase N catalytic chain, Cpn1 ELISA KIT
Packing:96T
Catalog No.:ELI-50279m
Gene Name:Mouse Cpn1
Detect Range:0.15-10ng/ml
Sensitivity:0.094ng/ml
Target Protein Name:Mouse Cpn1
Alternative Name:Mouse Carboxypeptidase N catalytic chain, Cpn1
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Mouse Carboxypeptidase N catalytic chain, Cpn1 ELISA KIT allows for the in vitro quantitative determination of Mouse Cpn1 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Mouse Carboxypeptidase N catalytic chain, Cpn1 ELISA KIT has been pre-coated with an Mouse Carboxypeptidase N catalytic chain, Cpn1 antibody specific to Mouse Cpn1 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Mouse Cpn1 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Mouse Cpn1 , biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Mouse Cpn1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
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