Mouse 3- hydroxyanthranilate 3,4- dioxygenase, Haao ELISA KIT Product Name:Mouse 3- hydroxyanthranilate 3,4- dioxygenase, Haao ELISA KIT
Packing:96T
Catalog No.:ELI-49757m
Gene Name:Mouse Haao
Detect Range:15.62-1000pg/ml
Sensitivity:9.375pg/ml
Target Protein Name:Mouse Haao
Alternative Name:Mouse 3-hydroxyanthranilate 3,4-dioxygenase, Haao
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Mouse 3- hydroxyanthranilate 3,4- dioxygenase, Haao ELISA KIT allows for the in vitro quantitative determination of Mouse Haao concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Mouse 3- hydroxyanthranilate 3,4- dioxygenase, Haao ELISA KIT has been pre-coated with an Mouse 3-hydroxyanthranilate 3,4-dioxygenase, Haao antibody specific to Mouse Haao .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Mouse Haao and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Mouse Haao , biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Mouse Haao in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
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