Mouse Acetolactate synthase- like protein, Ilvbl ELISA KIT
96 Tests
Operating instruction
FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!
Synonyms
2.2.1.-,Acetolactate synthase-like protein,IlvB-like protein, ILVBL,209L8,AHAS,ILV2H,acetolactate synthase homolog,acetolactate synthase-like protein,ilvB-like protein,ilvB (bacterial acetolactate synthase)-like
Search name
Mouse 2.2.1.- ELISA KIT ,Mouse Acetolactate synthase-like protein) ELISA KIT ,Mouse IlvB-like protein ELISA KIT ,Mouse ILVBL ELISA KIT ,Mouse 209L8 ELISA KIT ,Mouse AHAS ELISA KIT ,Mouse ILV2H ELISA KIT ,Mouse acetolactate synthase homolog ELISA KIT ,Mouse acetolactate synthase-like protein ELISA KIT ,Mouse ilvB-like protein ELISA KIT ,Mouse ilvB (bacterial acetolactate synthase)-like ELISA KIT
Intended use
This immunoassay kit allows for the in vitro quantitative determination of Mouse Acetolactate synthase-like protein concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.
Test principle
The microtiter plate provided in this kit has been pre-coated with an antibody specific to Acetolactate synthase-like protein. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for Acetolactate synthase-like protein and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Acetolactate synthase-like protein, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Acetolactate synthase-like protein in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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