Mouse Golgi apparatus protein 1, GLG1 ELISA KIT
96 Tests
Operating instruction
FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!
Synonyms
GLG1,CFR-1; ESL-1; MG-160; MG160; E-selectin ligand 1; cysteine-rich fibroblast growth factor receptor; golgi sialoglycoprotein MG-160; golgi glycoprotein 1, Golgi apparatus protein 1
Search name
Mouse GLG1 ELISA KIT , Mouse GLG ELISA KIT,Mouse CFR-1 ELISA KIT ,Mouse ESL-1 ELISA KIT ,Mouse MG-160 ELISA KIT ,Mouse MG160 ELISA KIT ,Mouse E-selectin ligand 1 ELISA KIT ,Mouse cysteine-rich fibroblast growth factor receptor ELISA KIT ,Mouse golgi sialoglycoprotein MG-160 ELISA KIT ,Mouse golgi glycoprotein 1 ELISA KIT ,Mouse Golgi apparatus protein 1 ELISA KIT, Mouse Golgi apparatus protein ELISA KIT
Intended use
This immunoassay kit allows for the in vitro quantitative determination of Mouse Golgi apparatus protein 1 concentrations in serum, Plasma,tissue homogenates and Cell culture supernates and Other biological fluids.
Test principle
The microtiter plate provided in this kit has been pre-coated with an antibody specific to Golgi apparatus protein 1. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific forGolgi apparatus protein 1 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Golgi apparatus protein 1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Golgi apparatus protein 1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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