Mouse Inter- alpha- trypsin inhibitor heavy chain H5, ITIH5 ELISA Kit
96 Tests
Operating instructions
FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!
Synonyms
ITIH5,ITI-HC5; ITI heavy chain H5; inter-alpha (globulin) inhibitor H5; inter-alpha-inhibitor heavy chain 5; inter-alpha-trypsin inhibitor heavy chain H5; inter-alpha-trypsin inhibitor heavy chain family, member 5,Inter-alpha-trypsin inhibitor heavy chain H5(ITI heavy chain H5;ITI-HC5;Inter-alpha-inhibitor heavy chain 5
Search name
Mouse ITIH5 ELISA KIT ,Mouse ITI-HC5 ELISA KIT ,Mouse ITI heavy chain H5 ELISA KIT ,Mouse inter-alpha (globulin) inhibitor H5 ELISA KIT ,Mouse inter-alpha-inhibitor heavy chain 5 ELISA KIT ,Mouse inter-alpha-trypsin inhibitor heavy chain H5 ELISA KIT ,Mouse inter-alpha-trypsin inhibitor heavy chain family member 5 ELISA KIT ,Mouse Inter-alpha-trypsin inhibitor heavy chain H5 ELISA KIT ,Mouse ITI heavy chain H5 ELISA KIT ,Mouse ITI-HC5 ELISA KIT ,Mouse Inter-alpha-inhibitor heavy chain 5 ELISA KIT
Intended use
This immunoassay kit allows for the in vitro quantitative determination of Mouse Inter- alpha- trypsin inhibitor heavy chain H5, ITIH5 concentrations in serum, plasma, tissue homogenates, cell culture supernates, and other biological fluids.
Test principle
The ELISA is based on the competitive binding enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Inter-alpha- trypsin inhibitor heavy chain H5, During the reaction, Inter-alpha- trypsin inhibitor heavy chain H5 in the sample or standard competes with a fixed amount of biotin-labeled Inter-alpha- trypsin inhibitor heavy chain H5 for sites on a pre-coated Monoclonal antibody specific to Inter-alpha- trypsin inhibitor heavy chain H5. Excess conjugate and unbound sample or standard are washed from the plate. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Inter-alpha- trypsin inhibitor heavy chain H5 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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