Mouse Carbamoyl- phosphate synthase [ammonia], mitochondrial, CPS1 ELISA KIT
96 Tests
Operating instruction
FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!
Synonyms
Carbamoyl-phosphate synthase [ammonia], mitochondrial),Carbamoyl-phosphate synthetase I,CPSase I,CPS1,CPSM,CPSASE1; PHN; carbamoyl-phosphate synthase [ammonia], mitochondrial; carbamoylphosphate synthetase I; carbamoyl-phosphate synthase 1 mitochondrial
Search name
Mouse Carbamoyl-phosphate synthase [ammonia] mitochondrial) ELISA KIT ,Mouse Carbamoyl-phosphate synthetase I ELISA KIT ,Mouse CPSase I ELISA KIT ,Mouse CPS1 ELISA KIT ,Mouse CPSM ELISA KIT ,Mouse CPSASE1 ELISA KIT ,Mouse PHN ELISA KIT ,Mouse carbamoylphosphate synthetase I ELISA KIT ,Mouse carbamoyl-phosphate synthase 1 mitochondrial ELISA KIT
Intended use
This immunoassay kit allows for the in vitro quantitative determination of Mouse carbamoyl-phosphate synthase [ammonia], mitochondrial,CPSM concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.
Test principle
The microtiter plate provided in this kit has been pre-coated with an antibody specific to CPSM. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for CPSM and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain CPSM, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of CPSM in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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