Mouse Protein kinase C- binding protein NELL1, NELL1 ELISA KIT
96 Tests
Operating instruction
FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!
Synonyms
Protein kinase C-binding protein NELL1,NEL-like protein 1,Nel-related protein 1, NELL1,protein kinase C-binding protein NELL1; IDH3GL; NRP1; NEL-like protein 1; NELL-1 protein short isoform; nel-related protein 1; neural epidermal growth factor-like 1; NEL-like 1
Search name
Mouse NELL1 ELISA KIT ,Mouse Protein kinase C-binding protein NELL1 ELISA KIT ,Mouse NEL-like protein 1 ELISA KIT ,Mouse Nel-related protein 1 ELISA KIT ,Mouse protein kinase C-binding protein NELL1 ELISA KIT ,Mouse IDH3GL ELISA KIT ,Mouse NRP1 ELISA KIT ,Mouse NEL-like protein 1 ELISA KIT ,Mouse NELL-1 protein short isoform ELISA KIT ,Mouse nel-related protein 1 ELISA KIT ,Mouse neural epidermal growth factor-like 1 ELISA KIT ,Mouse NEL-like 1 ELISA KIT
Intended use
Mouse NELL1 ELISA KIT allows for the in vitro quantitative determination of Mouse Protein kinase C-binding protein NELL1 concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.
Test principle
The microtiter plate provided in Mouse NELL1 ELISA KIT has been pre-coated with an antibody specific to Protein kinase C-binding protein NELL1. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for Protein kinase C-binding protein NELL1 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Protein kinase C-binding protein NELL1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Protein kinase C-binding protein NELL1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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