Mouse Serum amyloid A- 4 protein, SAA4 ELISA KIT
96 Tests
Operating instruction
FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!
Synonyms
Serum amyloid A-4 protein,Constitutively expressed serum amyloid A protein,SAA4,C-SAA; CSAA; constitutively expressed serum amyloid A protein; serum amyloid A-4 protein; serum amyloid A4 constitutive
Search name
Mouse Serum amyloid A-4 protein ELISA KIT ,Mouse Constitutively expressed serum amyloid A protein ELISA KIT ,Mouse SAA4 ELISA KIT ,Mouse C-SAA ELISA KIT ,Mouse CSAA ELISA KIT ,Mouse constitutively expressed serum amyloid A protein ELISA KIT ,Mouse serum amyloid A-4 protein ELISA KIT ,Mouse serum amyloid A4 constitutive ELISA KIT
Intended use
This immunoassay kit allows for the in vitro quantitative determination of Mouse serum amyloid a-4 protein,SAA4 concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.
Test principle
The microtiter plate provided in this kit has been pre-coated with an antibody specific to SAA4. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for SAA4 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain SAA4, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of SAA4 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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