Product Name:Mouse F- box only protein 25, Fbxo25 ELISA KIT
Packing:96T
Catalog No.:ELI-38493m
Gene Name:Mouse Fbxo25
Detect Range:0.156-10ng/ml
Sensitivity:0.094ng/ml
Target Protein Name:Mouse Fbxo25
Alternative Name:Mouse F-box only protein 25, Fbxo25
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Mouse F- box only protein 25, Fbxo25 ELISA KIT allows for the in vitro quantitative determination of Mouse Fbxo25 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Mouse F- box only protein 25, Fbxo25 ELISA KIT has been pre-coated with an Mouse F-box only protein 25, Fbxo25 antibody specific to Mouse Fbxo25 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Mouse Fbxo25 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Mouse Fbxo25 , biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Mouse Fbxo25 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
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