Mouse Type I iodothyronine deiodinase, DIO1 ELISA KIT
96 Tests
Operating instructions
FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!
Synonyms
DIO1,5DI; TXDI1; DIOI; thyroxine deiodinase type I selenoprotein; type 1 DI; type I iodothyronine deiodinase; type-I 5'-deiodinase; deiodinase iodothyronine, type I ,Type I iodothyronine deiodinase,Type-I 5'-deiodinase,Type 1 DI,5DI,DIOI,
Search name
Mouse DIO1 ELISA KIT ,Mouse 5DI ELISA KIT ,Mouse TXDI1 ELISA KIT ,Mouse DIOI ELISA KIT ,Mouse thyroxine deiodinase type I selenoprotein ELISA KIT ,Mouse type 1 DI ELISA KIT ,Mouse type I iodothyronine deiodinase ELISA KIT ,Mouse type-I 5'-deiodinase ELISA KIT ,Mouse deiodinase iodothyronine type I ELISA KIT ,Mouse Type I iodothyronine deiodinase ELISA KIT ,Mouse Type-I 5'-deiodinase ELISA KIT ,Mouse Type 1 DI ELISA KIT ,Mouse 5DI ELISA KIT ,Mouse DIOI ELISA KIT
Intended use
This immunoassay kit allows for the in vitro quantitative determination of Mouse type Type I iodothyronine deiodinase concentrations in serum, plasma, tissue homogenates, cell culture supernates, and other biological fluids.
Test principle
The ELISA is based on the competitive binding enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Type I iodothyronine deiodinase, During the reaction, Type I iodothyronine deiodinase in the sample or standard competes with a fixed amount of biotin-labeled Type I iodothyronine deiodinase for sites on a pre-coated Monoclonal antibody specific to Type I iodothyronine deiodinase. Excess conjugate and unbound sample or standard are washed from the plate. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Type I iodothyronine deiodinase in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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