Mouse Sarcosine dehydrogenase mitochondrial, SarDH ELISA Kit
96 Tests
Operating instructions
FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!
Synonyms
SarDH,BPR-2, sarcosine dehydrogenase,BPR-2; DMGDHL1; SAR; SARD; SDH; dimethylglycine dehydrogenase-like 1; sarcosine dehydrogenase mitochondrial
Search name
Mouse SarDH ELISA KIT ,Mouse BPR-2 ELISA KIT ,Mouse sarcosine dehydrogenase ELISA KIT ,Mouse BPR-2 ELISA KIT ,Mouse DMGDHL1 ELISA KIT ,Mouse SAR ELISA KIT ,Mouse SARD ELISA KIT ,Mouse SDH ELISA KIT ,Mouse dimethylglycine dehydrogenase-like 1 ELISA KIT ,Mouse sarcosine dehydrogenase mitochondrial ELISA KIT
Intended use
This immunoassay kit allows for the in vitro quantitative determination of mouse sarcosine dehydrogenase, mitochondrial,SarDH concentrations in serum, plasma, tissue homogenates, cell culture supernates, and other biological fluids.
Test principle
The ELISA is based on the competitive binding enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with an antibody specific to SarDH, During the reaction, SarDH in the sample or standard competes with a fixed amount of biotin-labeled SarDH for sites on a pre-coated Monoclonal antibody specific to SarDH. Excess conjugate and unbound sample or standard are washed from the plate. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of SarDH in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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