Mouse Protein patched homolog 2, PTCH2 ELISA Kit
96 Tests
Operating instructions
FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!
Synonyms
PTCH2,PTC2; patched homolog 2; patched 2, Protein patched homolog 2
Search name
Mouse PTCH2 ELISA KIT ,Mouse PTC2 ELISA KIT ,Mouse patched homolog 2 ELISA KIT ,Mouse patched 2 ELISA KIT ,Mouse Protein patched homolog 2 ELISA KIT
Intended use
This immunoassay kit allows for the in vitro quantitative determination of mouse PTC2 concentrations in serum, plasma, tissue homogenates, cell culture supernates, and other biological fluids.
Test principle
The ELISA is based on the competitive binding enzyme immunoassaytechnique. The microtiter plate provided in this kit has been pre-coated with an antibody specific toPTC2, During the reaction,PTC2in the sample or standard competes with a fixed amount of biotin-labeledPTC2 for sites on a pre-coated Monoclonal antibody specific to PTC2. Excess conjugate and unbound sample or standard are washed from the plate. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Thena TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of PTC2 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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