Mouse Ribonucleoside-diphosphate reductase subunit M2, RRM2 ELISA Kit
96 Tests
Operating instruction
FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!
Synonyms
RRM2,RR2; RR2M; ribonucleoside-diphosphate reductase subunit M2; ribonucleotide reductase M2 polypeptide; ribonucleotide reductase small chain; ribonucleotide reductase small subunit; ribonucleotide reductase M2,Ribonucleoside-diphosphate reductase subunit M2,Ribonucleotide reductase small chain,Ribonucleotide reductase small subunit,;
Search name
Mouse RRM2 ELISA KIT ,Mouse RR2 ELISA KIT ,Mouse RR2M ELISA KIT ,Mouse ribonucleoside-diphosphate reductase subunit M2 ELISA KIT ,Mouse ribonucleotide reductase M2 polypeptide ELISA KIT ,Mouse ribonucleotide reductase small chain ELISA KIT ,Mouse ribonucleotide reductase small subunit ELISA KIT ,Mouse ribonucleotide reductase M2 ELISA KIT ,Mouse Ribonucleoside-diphosphate reductase subunit M2 ELISA KIT ,Mouse Ribonucleotide reductase small chain ELISA KIT ,Mouse Ribonucleotide reductase small subunit ELISA KIT
Intended use
This immunoassay kit allows for the in vitro quantitative determination of Mouse ribonucleoside-diphosphate reductase subunit m2,RRM2 concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.
Test principle
The microtiter plate provided in this kit has been pre-coated with an antibody specific to RRM2. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for RRM2 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain RRM2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of RRM2 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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