Product Name:Mouse Gap junction alpha- 10 protein, Gja10 ELISA KIT
Packing:96T
Catalog No.:ELI-33591m
Gene Name:Mouse Gja10
Detect Range:0.156-10ng/ml
Sensitivity:0.094ng/ml
Target Protein Name:Mouse Gja10
Alternative Name:Mouse Gap junction alpha-10 protein, Gja10
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Mouse Gap junction alpha- 10 protein, Gja10 ELISA KIT allows for the in vitro quantitative determination of Mouse Gja10 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Mouse Gap junction alpha- 10 protein, Gja10 ELISA KIT has been pre-coated with an Mouse Gap junction alpha-10 protein, Gja10 antibody specific to Mouse Gja10 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Mouse Gja10 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Mouse Gja10 , biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Mouse Gja10 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
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