Mouse Voltage- dependent calcium channel subunit alpha- 2/ delta- 2, CACNA2D2 ELISA KIT
96 Tests
Operating instruction
FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!
Synonyms
CACNA2D2,CACNA2D; alpha 2 delta calcium channel subunit; gene 26; voltage-dependent calcium channel subunit alpha-2/delta-2; voltage-gated calcium channel subunit alpha-2/delta-2; calcium channel, voltage-dependent, alpha 2/delta subunit 2
Search name
Mouse CACNA2D2 ELISA KIT ,Mouse CACNA2D ELISA KIT ,Mouse alpha 2 delta calcium channel subunit ELISA KIT ,Mouse gene 26 ELISA KIT ,Mouse voltage-dependent calcium channel subunit alpha-2/delta-2 ELISA KIT ,Mouse voltage-gated calcium channel subunit alpha-2/delta-2 ELISA KIT ,Mouse calcium channel voltage-dependent alpha 2/delta subunit 2 ELISA KIT
Intended use
This immunoassay kit allows for the in vitro quantitative determination of Mouse Voltage- dependent calcium channel subunit alpha- 2/delta- 2, CACNA2D2 concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.
Test principle
The microtiter plate provided in this kit has been pre-coated with an antibody specific to CACNA2D2. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for CACNA2D2 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain CACNA2D2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of CACNA2D2 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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