Mouse DNA excision repair protein ERCC- 8, Ercc8 ELISA KIT Product Name:Mouse DNA excision repair protein ERCC- 8, Ercc8 ELISA KIT
Packing:96T
Catalog No.:ELI-32579m
Gene Name:Mouse Ercc8
Detect Range:78.125-5000pg/ml
Sensitivity:46.875pg/ml
Target Protein Name:Mouse Ercc8
Alternative Name:Mouse DNA excision repair protein ERCC-8, Ercc8
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Mouse DNA excision repair protein ERCC- 8, Ercc8 ELISA KIT allows for the in vitro quantitative determination of Mouse Ercc8 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Mouse DNA excision repair protein ERCC- 8, Ercc8 ELISA KIT has been pre-coated with an Mouse DNA excision repair protein ERCC-8, Ercc8 antibody specific to Mouse Ercc8 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Mouse Ercc8 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Mouse Ercc8 , biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Mouse Ercc8 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
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