Mouse Ephrin type-B receptor 4, EphB4 ELISA Kit
96 Tests
Operating instruction
FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!
Synonyms
Ephrin type-B receptor 4,Tyrosine kinase MYK-1,Developmental kinase 2,mDK-2,Tyrosine kinase MYK-1, EphB4,soluble EPHB4 variant 3; soluble EPHB4 variant 2; soluble EPHB4 variant 1; ephrin receptor EphB4; HTK; MYK1; TYRO11; ephrin type-B receptor 4; hepatoma transmembrane kinase; tyrosine-protein kinase TYRO11; tyrosine-protein kinase receptor HTK; EPH receptor B4
Search name
Mouse Ephrin type-B receptor 4 ELISA KIT ,Mouse Tyrosine kinase MYK-1 ELISA KIT ,Mouse Developmental kinase 2 ELISA KIT ,Mouse mDK-2 ELISA KIT ,Mouse Tyrosine kinase MYK-1 ELISA KIT ,Mouse EphB4 ELISA KIT ,Mouse soluble EPHB4 variant 3 ELISA KIT ,Mouse soluble EPHB4 variant 2 ELISA KIT ,Mouse soluble EPHB4 variant 1 ELISA KIT ,Mouse ephrin receptor EphB4 ELISA KIT ,Mouse HTK ELISA KIT ,Mouse MYK1 ELISA KIT ,Mouse TYRO11 ELISA KIT ,Mouse ephrin type-B receptor 4 ELISA KIT ,Mouse hepatoma transmembrane kinase ELISA KIT ,Mouse tyrosine-protein kinase TYRO11 ELISA KIT ,Mouse tyrosine-protein kinase receptor HTK ELISA KIT ,Mouse EPH receptor B4 ELISA KIT
Intended use
This immunoassay kit allows for the in vitro quantitative determination of mouse Ephrin type-B receptor 4 concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.
Test principle
The microtiter plate provided in this kit has been pre-coated with an antibody specific to Ephrin type-B receptor 4. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for Ephrin type-B receptor 4 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Ephrin type-B receptor 4, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Ephrin type-B receptor 4 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
PDF manual download
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