Mouse Large neutral amino acids transporter small subunit 1, SLC7A5 ELISA Kit
96 Tests
Operating instruction
FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!
Synonyms
SLC7A5,CD98 light chain; CD98; 4F2LC; D16S469E; E16; LAT1; MPE16; hLAT1; 4F2 LC; 4F2 light chain; L-type amino acid transporter 1; integral membrane protein E16; large neutral amino acids transporter 1; large neutral amino acids transporter small subunit 1; sodium-independent neutral amino acid transporter LAT1; solute carrier family 7 (cationic amino acid transporter, y+ system), member 5; solute carrier family 7 member 5; y+ system cationic amino acid transporter; solute carrier family 7 (amino acid transporter light chain, L system), member 5
Search name
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Intended use
This immunoassay kit allows for the in vitro quantitative determination of Mouse Large neutral amino acids transporter small subunit 1, SLC7A5 beta concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.
Test principle
The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse Large neutral amino acids transporter small subunit 1, SLC7A5. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for Mouse Large neutral amino acids transporter small subunit 1, SLC7A5 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Mouse Large neutral amino acids transporter small subunit 1, SLC7A5, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Mouse Large neutral amino acids transporter small subunit 1, SLC7A5 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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