Product Name:Mouse Golgi pH regulator, Gpr89a ELISA KIT
Packing:96T
Catalog No.:ELI-27862m
Gene Name:Mouse Gpr89a
Detect Range:31.25-2000pg/ml
Sensitivity:18.75pg/ml
Target Protein Name:Mouse Gpr89a
Alternative Name:Mouse Golgi pH regulator, Gpr89a
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Mouse Golgi pH regulator, Gpr89a ELISA KIT allows for the in vitro quantitative determination of Mouse Gpr89a concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Mouse Golgi pH regulator, Gpr89a ELISA KIT has been pre-coated with an Mouse Golgi pH regulator, Gpr89a antibody specific to Mouse Gpr89a .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Mouse Gpr89a and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Mouse Gpr89a , biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Mouse Gpr89a in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
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