Mouse Thiomorpholine- carboxylate dehydrogenase, CRYM ELISA KIT
96 Tests
Operating instruction
FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!
Synonyms
CRYM,DFNA40; THBP; NADP-regulated thyroid-hormone binding protein; ketimine reductase; mu-crystallin homolog; thiomorpholine-carboxylate dehydrogenase; crystallin, mu
Search name
Mouse CRYM ELISA KIT ,Mouse DFNA40 ELISA KIT ,Mouse THBP ELISA KIT ,Mouse NADP-regulated thyroid-hormone binding protein ELISA KIT ,Mouse ketimine reductase ELISA KIT ,Mouse mu-crystallin homolog ELISA KIT ,Mouse thiomorpholine-carboxylate dehydrogenase ELISA KIT ,Mouse crystallin mu ELISA KIT
Intended use
This immunoassay kit allows for the in vitro quantitative determination of Mouse Thiomorpholine- carboxylate dehydrogenase, CRYM concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.
Test principle
The microtiter plate provided in this kit has been pre-coated with an antibody specific to CRYM. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for CRYM and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain CRYM, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of CRYM in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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