Mouse 11- Beta Hydroxysteroid Dehydrogenase Type 1, HSD11B1 ELISA Kit
96 Tests
Operating instruction
FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE
BEGINNING!
Synonyms
Corticosteroid 11-beta-dehydrogenase isozyme 1, 11-beta-hydroxysteroid dehydrogenase 1(11-DH,11-beta-HSD1), 11-DH;
11-beta-HSD1; CORTRD2; HDL; HSD11; HSD11B; HSD11L; SDR26C1; corticosteroid 11-beta-dehydrogenase isozyme 1; short chain
dehydrogenase/reductase family 26C, member 1; hydroxysteroid (11-beta) dehydrogenase 1
Search name
Mouse Corticosteroid 11-beta-dehydrogenase isozyme 1 ELISA Kit, Mouse HSD11L ELISA Kit , Mouse 11-Beta Hydroxysteroid
Dehydrogenase Type 1 ELISA Kit, Mouse HSD11B1 ELISA Kit, Mouse 11-beta-hydroxysteroid dehydrogenase 1 ELISA Kit,
Mouse 11-DH ELISA Kit, Mouse 11-beta-HSD1 ELISA Kit, Mouse 11-DH ELISA Kit, Mouse CORTRD2 ELISA Kit, Mouse HDL
ELISA Kit, Mouse HSD11 ELISA Kit, Mouse HSD11B ELISA Kit, Mouse SDR26C1 ELISA Kit, Mouse reductase family 26C
member 1 ELISA Kit, Mouse short chain dehydrogenase member 1ELISA Kit, Mouse hydroxysteroid dehydrogenase 1 ELISA
Kit, Mouse 11-beta dehydrogenase 1 ELISA Kit
Intended use
This immunoassay kit allows for the in vitro quantitative determination of Mouse corticosteroid 11-beta-dehydrogenase isozyme
1,HSD11L concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.
Test principle
The microtiter plate provided in this kit has been pre-coated with an antibody specific to HSD11L. Standards or samples are then added to
the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for HSD11L and Avidin conjugated
to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well.
Only those wells that contain HSD11L, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The
enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured
spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of HSD11L in the samples is then determined by comparing
the O.D. of the samples to the standard curve.
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