Mouse Alpha- S1- casein, CSN1S1 ELISA KIT
96 Tests
Operating instruction
FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!
Synonyms
Alpha-S1-casein, CSN1S1,CASA; CSN1; alpha-S1-casein; casein, alpha; casein alpha s1
Search name
Mouse Alpha-S1-casein ELISA KIT ,Mouse CSN1S1 ELISA KIT ,Mouse CASA ELISA KIT ,Mouse CSN1 ELISA KIT ,Mouse alpha-S1-casein ELISA KIT ,Mouse casein alpha ELISA KIT ,Mouse casein alpha s1 ELISA KIT
Intended use
This immunoassay kit allows for the in vitro quantitative determination of Mouse Alpha-S1-casein concentrations in serum, Plasma, Urine, tissue homogenates and Cell culture supernates and Other biological fluids.
Test principle
The microtiter plate provided in this kit has been pre-coated with an antibody specific to Alpha-S1-casein. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for Alpha-S1-casein and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Alpha-S1-casein, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Alpha-S1-casein in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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